Affect of temperature on enzyme activity?

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-as temperature increases, the kinetic energy increases the molecules move faster 

-increase chance of successful collisions

-increase chance of forming ES complex

-increase rate of reaction

-carries on till optimum

-after optimum bonds in tertiary structure break (hydrogen and ionic bonds)

-lose active site shape

-substrate no longer complementary

-can't form ES complexes

-enzyme denatured

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