How do murder clonial antibodies identify specific antibodies in the blood?

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by inkhybrid

by inkhybrid

-e.g identify TB antibodies in the blood

-Please antigen complementary to TB antibodies on test plate

-Add persons blood to test plate

-If TB antibodies are present in blood, they will bind to the antigen

-Then a set of monoclonal antibodies [with an enzyme attached] complementary to the TB antibodies are added

-If the TB antibodies are present, the monoclonal antibodies will attach

-If the TB antibodies are not present the monoclonal antibodies will not attach

-The test plate is then washed

-If the TB antibodies are present, the monoclonal antibodies will attach this will not be washed away, so the enzyme will be present

-If the TB antibodies are not present, the monoclonal antibodies will not attach, this will be washed away, so the enzyme will be washed away

-A colorless substrate is then added, if the enzyme is present it will breakdown the substrate causing a color change, if the enzyme is not present there will be no color change.

-therefore: color change occurs = enzyme present/ TB antibody is present, no color change = no enzyme present/ no TB antibody is present.