Asked by nooraqilahrusli (How do you remember your username? I had to copy and paste.)
A Knot is when the base of an Alpha's penis (or in a female's case: engorged clitoris) swells and locks in an omega's uterus. Basically, when they're about to cum, blood flows to the base to lock them in and they're stuck until the blood moves on or they're completely finished.
There is science behind it, it acts just like a dog's knot.
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Omegaverse guide
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